polyclonal rabbit anti ido1 antibody  (Proteintech)

Journal: BMC microbiology

Article Title: Lactobacillus Johnsonii YH1136 alleviates schizophrenia-like behavior in mice: a gut-microbiota-brain axis hypothesis study.

doi: 10.1186/s12866-025-03893-w

Figure Lengend Snippet: Fig. 2 The expression of related proteins in the hippocampus and prefrontal cortex (PFC) of mice. mRNA expression level of BDNF (a), TDO2 (b), IDO1 (c), IDO2 (d), KAT1 (e), KAT2 (f), KAT4 (g), KAT4 (h), TPH1 (i), NR1 (j), NR2A (k), and NR2B (l) in the Hippocampus and PFC. Data are expressed as mean ± SD (n = 5 per group). ns no significant, *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: Sections were then incubated overnight at 4 °C with polyclonal rabbit anti-TDO2 antibody (1:100, 15,880–1-AP, Proteintech), polyclonal rabbit anti-IDO1 antibody (1:100, 13,268–1-AP, Proteintech), polyclonal rabbit anti-IDO2 antibody (1:200, bs-16640R, Bioss), polyclonal rabbit anti-TPH1 antibody (1:50, BS3727, Bioworld), or polyclonal rabbit anti-KAT1 antibody (1:500, GB11906, Servicebio).

Techniques: Expressing

Journal: BMC microbiology

Article Title: Lactobacillus Johnsonii YH1136 alleviates schizophrenia-like behavior in mice: a gut-microbiota-brain axis hypothesis study.

doi: 10.1186/s12866-025-03893-w

Figure Lengend Snippet: Fig. 3 Immunofluorescence staining. The sections of the TDO2 (a-b), IDO1 (c-d), IDO2 (e–f), KAT1 (g-h), TPH1 (i-j) in hippocampus and PFC. The magnification is 20X (F)

Article Snippet: Sections were then incubated overnight at 4 °C with polyclonal rabbit anti-TDO2 antibody (1:100, 15,880–1-AP, Proteintech), polyclonal rabbit anti-IDO1 antibody (1:100, 13,268–1-AP, Proteintech), polyclonal rabbit anti-IDO2 antibody (1:200, bs-16640R, Bioss), polyclonal rabbit anti-TPH1 antibody (1:50, BS3727, Bioworld), or polyclonal rabbit anti-KAT1 antibody (1:500, GB11906, Servicebio).

Techniques: Immunofluorescence, Staining

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: Downregulation of tryptophan metabolism in asthma (A–C) The levels of cytokines IFN-γ (A), IL-4 (B), and IL-13 (C) in the serum between control and asthma group. Data are represented as mean ± SEM. See also Figure S1 A. (D and E) Serum levels of tryptophan (D) and kynurenine (E) in 10 health and 17 asthma participants were detected by mass spectrometry. (F) The ratio of Kyn to Trp is presented. Data are represented as mean ± SEM. (G) Correlation between Kyn/Trp and T1/T2 (IFN-γ/IL4+IL13) is shown. (H) The representative detail of IDO1 IHC staining images in asthma and matched non-asthma lung tissue. Scale bar, 100 μm. See also Figure S1 B. (I) The expression IDO1 in airway epithelium in mice models was detected by IHC. Scale bar, 100 μm. (J and K) Western blots (J) and semi-quantitative analysis (K) showed IDO1 levels. (L) The mRNA level of IDO1 in lung was detected by RT-PCR. One of three repeated experiments with similar results is presented. The animal experiments included 6–7 BALB/c mice in each group. ∗ p < 0.05, ∗∗ p < 0.01, ns, not significant. See also Figures S1 and and Tables S1 and .

Article Snippet: Rabbit polyclonal anti- IDO1 , Cell Signaling Technology , Cat#3879S; RRID: AB_2255011.

Techniques: Control, Mass Spectrometry, Immunohistochemistry, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: T1/2 cytokines regulated tryptophan metabolism to kynurenine in airway epithelium (A) The heatmap of relative mRNA levels (log10) of key enzymes (IDO1, IDO2, TDO2, and IL-4I1) in BEAS-2B cells treated with different cytokines (IFN-γ, TGF-β, IL-4, IL-5, and IL-13; all cytokines at 10 ng/mL) at different time points (4, 8, 12, 20, 24, 48 h) is presented. (B) Heatmap of metabolites related to tryptophan metabolism detected in BEAS-2B with or without 10 ng/mL IFN-γ for 24 h is shown. n = 4. See also Figure S2 C. (C and D) The concentration of Trp (C) and Kyn (D) were detected after stimulation with different concentrations of IFN-γ. Data are represented as mean ± SEM. (E) The ratio of Kyn to Trp after stimulation with different concentrations of IFN-γ is presented. Data are represented as mean ± SEM. (F and G) The concentration of Trp (C) and Kyn (D) were detected after stimulation with T2 cytokines (IL-4, IL-5, and IL13) at the presence of IFN-γ. Data are represented as mean ± SEM. (H) The ratio of Kyn to Trp was calculated after treatment with T2 cytokines at the presence of IFN-γ. Data are represented as mean ± SEM. ∗∗ p < 0.05, ∗∗∗∗ p < 0.01, ns, not significant. See also Figure S2 and Tables S3 and .

Article Snippet: Rabbit polyclonal anti- IDO1 , Cell Signaling Technology , Cat#3879S; RRID: AB_2255011.

Techniques: Concentration Assay

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: IDO1 is the key enzyme regulated by T1/2 cytokines in airway epithelial cells (A) Relative mRNA level of IDO1 in BEAS-2B cells was measured after 12 h - treatment with IFN-γ at 0, 0.01, 0.1, 1 ng/mL, 10 ng/mL by RT-PCR. Data are represented as mean ± SEM. (B) IDO1 protein level was detected by western blot in BEAS-2B cells 24 and 48 h after treatment with IFN-γ at 0, 2.5, 5, 7.5, 10 ng/mL. (C and D) The expression of IDO1 at transcription (C) and expression (D) levels at different time points after IFN-γ stimulation at 100 pg/mL in BEAS-2B cells. Data are represented as mean ± SEM. (E and F) IDO1 mRNA levels after 12 h (E) and protein level after 24 h (F) in BEAS-2B after stimulation of 10 ng/mL IL-4, IL-5, and IL-13 at the presence of 100 pg/mL IFN-γ. Data are represented as mean ± SEM. (G) IDO1 mRNA levels in BEAS-2B (pretreated with si IDO1 or control) after stimulation of 10 ng/mL IFN-γ. Data are represented as mean ± SEM. (H) IDO1 expression was detected by RT-PCR after 12 h—treatment with or without Ruxolitinib (5 μM) in the presence of IFN-γ (10 ng/mL). Data are represented as mean ± SEM. (I) Western blots showed IDO1 and JAK-STAT pathway proteins level in BEAS-2B cells after 24 h stimulation of IFN-γ (10 ng/mL) with or without Ruxolitinib (5 μM). Rux, Ruxolitinib, JAK1/2 inhibitor. Data in A, C, E, and G are means ± SEM. One-way analysis of variance (ANOVA) was performed in A, C, E, and G. One of three experiments with similar results is presented. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. See also Figure S4 .

Article Snippet: Rabbit polyclonal anti- IDO1 , Cell Signaling Technology , Cat#3879S; RRID: AB_2255011.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Control

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: Kyn induces AhR translocation to regulate the transcription of the CTH gene (A) ChIP-RT-PCR of CYP1B 1 and CTH with the AHRE-related primer was performed. The amount of immunoprecipitated DNA in each sample was represented as a signal relative to the total amount of input chromatin, normalized to one. Data are represented as mean ± SEM. See also Figure S5 B. (B) IDO1 and CTH protein levels in BEAS-2B (pretreated with overexpression IDO1 plasmid or control). (C) CTH mRNA levels in BEAS-2B (pretreated with si IDO1 or control). Data are represented as mean ± SEM. (D) Under the stimulation of high concentration of IFN-γ (10 ng/ml), the expression of CTH was detected by RT-PCR at different time points. Data are represented as mean ± SEM. (E) The expression of CTH and IDO1 were detected by western Blot with the stimulation of different concentration of IFN-γ after 48 h. (F) Under the stimulation of IFN-γ or Kyn, the expression of CTH was detected by RT-PCR. Data are represented as mean ± SEM. (G) The expression of CTH and IDO1 were detected by western Blot after stimulation of 10 ng/mL IL-4, IL-5, IL-13 in the presence of 100 pg/mL IFN-γ, respectively. (H) The mRNA levels of Cth in lung were detected. Data are represented as mean ± SEM. (I) The expression of CTH was detected by western blot with the treatment of IFN-γ, Kyn, IDO/TDO inhibitors, and AhR antagonist. IACS: IDO1 & TDO2 inhibitor; LM10: TDO2 inhibitor; CH223191, AhR antagonist. Data are means ± SEM. ∗∗∗ p < 0.001. One of three repeated experiments with similar results is presented. See also Figures S5 and .

Article Snippet: Rabbit polyclonal anti- IDO1 , Cell Signaling Technology , Cat#3879S; RRID: AB_2255011.

Techniques: Translocation Assay, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Over Expression, Plasmid Preparation, Control, Concentration Assay, Expressing, Western Blot

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: IDO1 overexpression relieved HDM-induced allergic asthma (A) Representative pathological images of lung tissues by HE staining are shown. (B) Airway and vascular inflammations were assessed. # on the column was the comparison between the control and asthma group, and ∗ on the line was the comparison of the AAV-control and AAV- Ido1 group. Data are represented as mean ± SEM. (C) Subepithelial fibrosis by Sirius Red staining is shown. (D) Mucus secretion by PAS staining is shown. (E) The expression of CTH was detected by IHC. (F) The mRNA level of CTH in lung was measured by RT-PCR. Data are represented as mean ± SEM. #### p < 0.0001, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant. One of three repeated experiments with similar results is presented. The animal experiments included 6–7 BALB/c mice in each group. Scale bar, 100 μm. See also Figure S7 .

Article Snippet: Rabbit polyclonal anti- IDO1 , Cell Signaling Technology , Cat#3879S; RRID: AB_2255011.

Techniques: Over Expression, Staining, Comparison, Control, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti- IDO1 , Cell Signaling Technology , Cat#3879S; RRID: AB_2255011.

Techniques: Virus, Recombinant, Plasmid Preparation, shRNA, Software

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: Downregulation of tryptophan metabolism in asthma (A–C) The levels of cytokines IFN-γ (A), IL-4 (B), and IL-13 (C) in the serum between control and asthma group. Data are represented as mean ± SEM. See also Figure S1 A. (D and E) Serum levels of tryptophan (D) and kynurenine (E) in 10 health and 17 asthma participants were detected by mass spectrometry. (F) The ratio of Kyn to Trp is presented. Data are represented as mean ± SEM. (G) Correlation between Kyn/Trp and T1/T2 (IFN-γ/IL4+IL13) is shown. (H) The representative detail of IDO1 IHC staining images in asthma and matched non-asthma lung tissue. Scale bar, 100 μm. See also Figure S1 B. (I) The expression IDO1 in airway epithelium in mice models was detected by IHC. Scale bar, 100 μm. (J and K) Western blots (J) and semi-quantitative analysis (K) showed IDO1 levels. (L) The mRNA level of IDO1 in lung was detected by RT-PCR. One of three repeated experiments with similar results is presented. The animal experiments included 6–7 BALB/c mice in each group. ∗ p < 0.05, ∗∗ p < 0.01, ns, not significant. See also Figures S1 and and Tables S1 and .

Article Snippet: Rabbit polyclonal anti- IDO1 , Proteintech , Cat#13268-1-AP; RRID: AB_2123444.

Techniques: Control, Mass Spectrometry, Immunohistochemistry, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: T1/2 cytokines regulated tryptophan metabolism to kynurenine in airway epithelium (A) The heatmap of relative mRNA levels (log10) of key enzymes (IDO1, IDO2, TDO2, and IL-4I1) in BEAS-2B cells treated with different cytokines (IFN-γ, TGF-β, IL-4, IL-5, and IL-13; all cytokines at 10 ng/mL) at different time points (4, 8, 12, 20, 24, 48 h) is presented. (B) Heatmap of metabolites related to tryptophan metabolism detected in BEAS-2B with or without 10 ng/mL IFN-γ for 24 h is shown. n = 4. See also Figure S2 C. (C and D) The concentration of Trp (C) and Kyn (D) were detected after stimulation with different concentrations of IFN-γ. Data are represented as mean ± SEM. (E) The ratio of Kyn to Trp after stimulation with different concentrations of IFN-γ is presented. Data are represented as mean ± SEM. (F and G) The concentration of Trp (C) and Kyn (D) were detected after stimulation with T2 cytokines (IL-4, IL-5, and IL13) at the presence of IFN-γ. Data are represented as mean ± SEM. (H) The ratio of Kyn to Trp was calculated after treatment with T2 cytokines at the presence of IFN-γ. Data are represented as mean ± SEM. ∗∗ p < 0.05, ∗∗∗∗ p < 0.01, ns, not significant. See also Figure S2 and Tables S3 and .

Article Snippet: Rabbit polyclonal anti- IDO1 , Proteintech , Cat#13268-1-AP; RRID: AB_2123444.

Techniques: Concentration Assay

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: IDO1 is the key enzyme regulated by T1/2 cytokines in airway epithelial cells (A) Relative mRNA level of IDO1 in BEAS-2B cells was measured after 12 h - treatment with IFN-γ at 0, 0.01, 0.1, 1 ng/mL, 10 ng/mL by RT-PCR. Data are represented as mean ± SEM. (B) IDO1 protein level was detected by western blot in BEAS-2B cells 24 and 48 h after treatment with IFN-γ at 0, 2.5, 5, 7.5, 10 ng/mL. (C and D) The expression of IDO1 at transcription (C) and expression (D) levels at different time points after IFN-γ stimulation at 100 pg/mL in BEAS-2B cells. Data are represented as mean ± SEM. (E and F) IDO1 mRNA levels after 12 h (E) and protein level after 24 h (F) in BEAS-2B after stimulation of 10 ng/mL IL-4, IL-5, and IL-13 at the presence of 100 pg/mL IFN-γ. Data are represented as mean ± SEM. (G) IDO1 mRNA levels in BEAS-2B (pretreated with si IDO1 or control) after stimulation of 10 ng/mL IFN-γ. Data are represented as mean ± SEM. (H) IDO1 expression was detected by RT-PCR after 12 h—treatment with or without Ruxolitinib (5 μM) in the presence of IFN-γ (10 ng/mL). Data are represented as mean ± SEM. (I) Western blots showed IDO1 and JAK-STAT pathway proteins level in BEAS-2B cells after 24 h stimulation of IFN-γ (10 ng/mL) with or without Ruxolitinib (5 μM). Rux, Ruxolitinib, JAK1/2 inhibitor. Data in A, C, E, and G are means ± SEM. One-way analysis of variance (ANOVA) was performed in A, C, E, and G. One of three experiments with similar results is presented. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. See also Figure S4 .

Article Snippet: Rabbit polyclonal anti- IDO1 , Proteintech , Cat#13268-1-AP; RRID: AB_2123444.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Control

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: Kyn induces AhR translocation to regulate the transcription of the CTH gene (A) ChIP-RT-PCR of CYP1B 1 and CTH with the AHRE-related primer was performed. The amount of immunoprecipitated DNA in each sample was represented as a signal relative to the total amount of input chromatin, normalized to one. Data are represented as mean ± SEM. See also Figure S5 B. (B) IDO1 and CTH protein levels in BEAS-2B (pretreated with overexpression IDO1 plasmid or control). (C) CTH mRNA levels in BEAS-2B (pretreated with si IDO1 or control). Data are represented as mean ± SEM. (D) Under the stimulation of high concentration of IFN-γ (10 ng/ml), the expression of CTH was detected by RT-PCR at different time points. Data are represented as mean ± SEM. (E) The expression of CTH and IDO1 were detected by western Blot with the stimulation of different concentration of IFN-γ after 48 h. (F) Under the stimulation of IFN-γ or Kyn, the expression of CTH was detected by RT-PCR. Data are represented as mean ± SEM. (G) The expression of CTH and IDO1 were detected by western Blot after stimulation of 10 ng/mL IL-4, IL-5, IL-13 in the presence of 100 pg/mL IFN-γ, respectively. (H) The mRNA levels of Cth in lung were detected. Data are represented as mean ± SEM. (I) The expression of CTH was detected by western blot with the treatment of IFN-γ, Kyn, IDO/TDO inhibitors, and AhR antagonist. IACS: IDO1 & TDO2 inhibitor; LM10: TDO2 inhibitor; CH223191, AhR antagonist. Data are means ± SEM. ∗∗∗ p < 0.001. One of three repeated experiments with similar results is presented. See also Figures S5 and .

Article Snippet: Rabbit polyclonal anti- IDO1 , Proteintech , Cat#13268-1-AP; RRID: AB_2123444.

Techniques: Translocation Assay, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Over Expression, Plasmid Preparation, Control, Concentration Assay, Expressing, Western Blot

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet: IDO1 overexpression relieved HDM-induced allergic asthma (A) Representative pathological images of lung tissues by HE staining are shown. (B) Airway and vascular inflammations were assessed. # on the column was the comparison between the control and asthma group, and ∗ on the line was the comparison of the AAV-control and AAV- Ido1 group. Data are represented as mean ± SEM. (C) Subepithelial fibrosis by Sirius Red staining is shown. (D) Mucus secretion by PAS staining is shown. (E) The expression of CTH was detected by IHC. (F) The mRNA level of CTH in lung was measured by RT-PCR. Data are represented as mean ± SEM. #### p < 0.0001, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significant. One of three repeated experiments with similar results is presented. The animal experiments included 6–7 BALB/c mice in each group. Scale bar, 100 μm. See also Figure S7 .

Article Snippet: Rabbit polyclonal anti- IDO1 , Proteintech , Cat#13268-1-AP; RRID: AB_2123444.

Techniques: Over Expression, Staining, Comparison, Control, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: iScience

Article Title: Impaired tryptophan metabolism by type 2 inflammation in epithelium worsening asthma

doi: 10.1016/j.isci.2024.109923

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti- IDO1 , Proteintech , Cat#13268-1-AP; RRID: AB_2123444.

Techniques: Virus, Recombinant, Plasmid Preparation, shRNA, Software

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: TDO, IDO1 and AhR expression in HUVEC and ECFC. ( A ) Real-time PCR analysis (mean ± SEM, n = 3). ( B , C ) Immunofluorescence, mean ± SEM, n = 3 and representative photomicrographs at 40× magnification for TDO (red) and IDO1 (green). ( D , E ) AhR (green) localization in the nucleus following the pro-angiogenic stimulus VEGF-A. Histograms show AhR fluorescence nuclear localization (AhR/DAPI) by Mander’s coefficient (M1) using Image J 1.54d software.

Article Snippet: Then, the following primary antibodies were added for overnight incubation at 4 °C: the monoclonal mouse anti human-TDO (1:200; Novus Biologicals, Briarwood Ave, OH, USA) or the polyclonal rabbit anti-human IDO1 (1:200; Abcam, Cambridge, UK) or the monoclonal rabbit anti-human AhR (1:100; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Fluorescence, Software

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: Effect of TDO and IDO1 inhibitors on in vitro angiogenesis. Angiogenesis was measured by capillary morphogenesis at 6 h in untreated HUVECs (ctr) and cells stimulated with 20 ng/mL VEGF-A in the absence or presence of 680C91 and epacadostat. ( A ) Representative pictures, 10× magnification. ( B ) Mean ± SEM, n = 3. § p < 0.05; §§ p < 0.04; §§§ p < 0.001 vs. untreated (ctr). *** p < 0.001 vs. VEGF-A.

Article Snippet: Then, the following primary antibodies were added for overnight incubation at 4 °C: the monoclonal mouse anti human-TDO (1:200; Novus Biologicals, Briarwood Ave, OH, USA) or the polyclonal rabbit anti-human IDO1 (1:200; Abcam, Cambridge, UK) or the monoclonal rabbit anti-human AhR (1:100; Cell Signaling Technology, Danvers, MA, USA).

Techniques: In Vitro

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: Effect of TDO and IDO1 inhibitors on in vitro vasculogenesis. Vasculogenesis was measured by capillary morphogenesis at 24 h in untreated ECFCs (ctr) and cells stimulated with 20 ng/mL VEGF-A in the absence or presence of 680C91 and epacadostat. ( A ) Representative pictures. 10× magnification. ( B ) Mean ± SEM, n = 3; § p < 0.05; §§ p < 0.01 vs. untreated (ctr). * p < 0.05; ** p < 0.01; *** p < 0.001 vs. VEGF-A.

Article Snippet: Then, the following primary antibodies were added for overnight incubation at 4 °C: the monoclonal mouse anti human-TDO (1:200; Novus Biologicals, Briarwood Ave, OH, USA) or the polyclonal rabbit anti-human IDO1 (1:200; Abcam, Cambridge, UK) or the monoclonal rabbit anti-human AhR (1:100; Cell Signaling Technology, Danvers, MA, USA).

Techniques: In Vitro

Journal: Pharmaceuticals

Article Title: Unveiling the Role of Tryptophan 2,3-Dioxygenase in the Angiogenic Process

doi: 10.3390/ph17050558

Figure Lengend Snippet: Effect of the TDO inhibitor 680C31 and IDO1 inhibitor epacadostat on HUVEC proliferation. Cell proliferation was evaluated in response to ( A , C ) VEGF-A and ( B , D ) FGF-2 in the presence of increasing concentrations of 680C91 ( A , B ). ( C , D ) Effects of the highest concentration of 680C91 (40 µM) and epacadostat (1 µM) on VEGF-A- and FGF-2-induced HUVEC growth. Mean ± SEM, n = 5; * p < 0.05 vs. VEGF-A alone; ** p < 0.01 vs. FGF-2 alone.

Article Snippet: Then, the following primary antibodies were added for overnight incubation at 4 °C: the monoclonal mouse anti human-TDO (1:200; Novus Biologicals, Briarwood Ave, OH, USA) or the polyclonal rabbit anti-human IDO1 (1:200; Abcam, Cambridge, UK) or the monoclonal rabbit anti-human AhR (1:100; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Concentration Assay

Journal: Biomolecules

Article Title: Expression of IDO1 and PD-L2 in Patients with Benign Lymphadenopathies and Association with Autoimmune Diseases.

doi: 10.3390/biom13020240

Figure Lengend Snippet: Figure 2. IDO1 in benign lymphadenopathies (rabbit polyclonal antibody HPA023149 (Atlas Antibod- ies AB, Stockholm, Sweden)) from patients with autoimmune diseases (C,F) and without autoimmune diseases (A,B,D,E). (A,B) Lymph node with high amounts of IDO1+ cells in both follicular (20%) and interfollicular (20%) areas (magnification 40× and 400×, respectively). (C) Low amount of follicular (0%) and intermediate amount of interfollicular (5%) IDO1+ cells (magnification 40×). (D) Intermediate amount of interfollicular (5%) and high amount of follicular (10%) IDO1+ cells in one germinal center, with no IDO1+ cells in other germinal centers (magnification 40×). (E) Low amounts of IDO1+ cells in both follicular (0%) and interfollicular (2%) areas (magnification 100×). (F) High amount of follicular (5%) and a very high amount of interfollicular (75%) IDO1+ cells. Note also endothelial IDO1+ cells (magnification 100×). IDO1+ leukocytes = black arrow, follicular areas = red arrow, IDO1+ endothelial cells = green arrow.

Article Snippet: Rabbit polyclonal antibody HPA023149 (Atlas Antibodies AB, Stockholm, Sweden) (dilu- tion 1:200) was used for IDO1, and rabbit monoclonal antibody D7U8C/82723 (Cell Sig- naling Technology, Danvers, MA) (dilution 1:50) was used for PD-L2.

Techniques: